| Group | Club/Team Name | Team ID | Head Coach |
| A1 | FC National - 01 FC National - B12U |
0291-01SJB2U-0003 | C Carroll - DO NOT USE |
| A2 | Chattanooga FC Academy Chattanooga FC Academy 2009 Gray Boys |
0316-029B2U-7790 | Dillon Thornbury |
| A3 | Rhea County Football Club Play (RC3) Rc3 FC Royals Boys U12 |
0322-01SJB2U-0016 | Jeremy Toliver |
| A4 | Tri-Cities United Soccer Club Tri-Cities United 2009 Boys Select |
0106-01AB2U-0009 | Nicolas Boegemann |
| A5 | Blount United Soccer Club Blount United - B12U Blue |
0201-01AB2U-0232 | A Castaneda |